BIOS 242 Week 1 iLab: Exploring the Microbiology Lab
Section 1 Lab Safety:
- There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts)
- Identify one of the three ways to keep your work area safe. (1 pt)
- There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt)
- There are several safety tips to protect one’s mucous membranes and broken skin. Identify one of the recommended tips. (1 pt)
- When working in a microbiology lab, it is important to contain spills and discard materials properly. Describe how a spill should be cleaned up safely in a microbiology lab. (3 pts)
- Describe the procedures that one must do before leaving the lab. (3 pts)
Section 2 Biochemical Tests:
- How do you know that no contamination occurred when you inoculated your culture? (1 pt)
- If the biochemical test requires an incubation of 24 hours, what do you need to do? (1 pt)
- If your biochemical test requires that a reagent is added, which tool do you need to use? (1 pt)
- There are two ways to record the results of a biochemical test. Watch the video found in the Recording Results tutorial. What was the result of the acid from glucose test? (1 pt)
- An important tool available in the Virtual Unknown program is the Identification Matrix. From the portion of the identification matrix shown in the Identifying Bacteria tutorial, identify at least one bacterium that has a positive result to the arabinose fermentation test. (1 pt)
Section 3: Reference Books:
- The first step of identification of an unknown bacterium is determining if the bacterium is gram negative or gram positive. (6 pts)
- What is the purpose of the dulcitol fermentation test? (1 pt)
- Which medium is used to perform the dulcitol fermentation test? What is the indicator used in this medium? (2 pts)
- After incubating the inoculated medium for 24 hours, the medium is yellow. Can the bacterium that you tested ferment dulcitol? (1 pt)
- Medium containing phenol red will be yellow when the pH is less than 6.8. Why would the medium change from a neutral pH to an acidic pH? (3 pts)
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